Effects of D-Glutamate on Enzymes of Ammonia Assimilation in Bacillus megaterium NCIB 7581

Abstract

SUMMARY: Enzymes possibly concerned in the assimilation of ammonia were assayed in Bacillus megaterium NCIB 7581 after growth with ammonium sulphate as sole source of nitrogen. Alanine dehydrogenase and aspartase were not detected, but both NADPH-dependent glutamate dehydrogenase and glutamate synthase (NADH- or NADPH-dependent) were found. Glutamine synthetase was also present, though differing methods of assay gave different values for its activity.