BIOCHEMISTRY OF THE EYE
- 1 May 1954
- journal article
- research article
- Published by American Medical Association (AMA) in A.M.A. Archives of Ophthalmology
- Vol. 51 (5), 589-592
- https://doi.org/10.1001/archopht.1954.00920040599002
Abstract
NO DETAILED analysis of the free amino acid content of the vitreous body has been reported. Duke-Elder1reported a total value, and Krause2determined the basic amino acids of the vitrein and mucoid portions of the vitreous. More recently Schaeffer and Murray3recorded the results of microbiological assays of the whole vitreous protein. In view of the paucity of detailed knowledge of the amino acids in the vitreous and their role in the chemical structure of that body, this report presents results of ion exchange chromatographic analysis of the amino acids which occur free in the vitreous humor. MATERIALS AND METHODS Bovine eyes were obtained from a meat-packing plant. Within four to eight hours after removal of the orbs from the freshly killed animal, the vitreous was separated and filtered by gravity. The filtered liquid was stored for short periods at 4 C. and in the frozenKeywords
This publication has 4 references indexed in Scilit:
- A CHROMATOGRAPHIC INVESTIGATION OF THE AMINO ACID CONSTITUENTS OF NORMAL URINEJournal of Biological Chemistry, 1953
- CHROMATOGRAPHY OF AMINO ACIDS ON SULFONATED POLYSTYRENE RESINSJournal of Biological Chemistry, 1951
- PHOTOMETRIC NINHYDRIN METHOD FOR USE IN THE CHROMATOGRAPHY OF AMINO ACIDSJournal of Biological Chemistry, 1948
- CHEMISTRY OF THE VITREOUS HUMORArchives of Ophthalmology (1950), 1934