Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella flexneri.
- 1 December 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (23), 9317-9321
- https://doi.org/10.1073/pnas.85.23.9317
Abstract
The large invasion plasmid of Shigella flexneri M9OT-W was used to generate recombinant plasmids carrying the ipaA, -B, -C, and -D genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. Complete DNA sequences of ipaB, -C, and -D were determined. The proteins predicted (62, 42, and 37 kDa, respectively) from the nucleotide sequences lack a signal-peptide sequence. Hydrophilic segments of the IpaB and IpaC proteins were found to overlap known epitopic domains of these membrane antigens. Analysis of total RNA demonstrated that temperature control of ipa gene expression occurs at the level of transcription. Multiple mRNA bands were detected by using ipa gene fragments as hybridization probes, and a putative transcript map for the ipa genes was constructed. Comparison of this map with the DNA sequence reveals a complex system of ipa gene regulation.This publication has 35 references indexed in Scilit:
- Protein unfolding and the energetics of protein translocation across biological membranesCell, 1988
- Processed mRNA with differential stability in the regulation of E. coli pilin gene expressionCell, 1988
- Localization of Plasmid Loci Necessary for the Entry of Shigella flexneri into HeLa Cells, and Characterization of One Locus Encoding Four Immunogenic PolypeptidesMicrobiology, 1987
- Identification of invasin: A protein that allows enteric bacteria to penetrate cultured mammalian cellsCell, 1987
- MECHANISM AND CONTROL OF TRANSCRIPTION INITIATION IN PROKARYOTESAnnual Review of Biochemistry, 1985
- Molecular Biology of Fimbriae of Enterotoxigenic Escherichia coliPublished by Springer Nature ,1985
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- GENETIC ANALYSIS OF THE MAJOR OUTER MEMBRANE PROTEINS OF ESCHERICHIA COLIAnnual Review of Genetics, 1981
- The Attachment to, and Invasion of HeLa Cells by Salmonella typhimurium: The Contribution of Mannose-sensitive and Mannoseresistant Haemagglutinating ActivitiesMicrobiology, 1981
- Prediction of protein antigenic determinants from amino acid sequences.Proceedings of the National Academy of Sciences, 1981