Change in the Proportion of Two Aspartokinases in Carrot Root Tissue in Response to in Vitro Culture
Open Access
- 1 January 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 61 (1), 115-118
- https://doi.org/10.1104/pp.61.1.115
Abstract
Two isofunctional aspartokinases (EC 2.7.2.4) exist in fresh root tissue of carrot (Daucus carota, cv. Oogata sanzun). The threoninesensitive portion constitutes about 70% of the activity; the lysinesensitive, less than 20%. Culture of slices of carrot tissue for 3 days reversed the ratio as the lysine-sensitive activity preferentially increased. Inhibition by threonine and lysine was additive in both enzyme preparations from fresh and cultured tissues. The activities were resolved into two distinct fractions of different sensitivity to threonine and lysine by DEAE-Sephadex A-50 column chromatography.This publication has 6 references indexed in Scilit:
- Properties and Regulation of Aspartate Kinase from Barley Seedlings (Hordeum vulgare L.)Plant Physiology, 1977
- Aspartokinase from Wheat GermPlant Physiology, 1973
- Aspartokinase in Lemna minor LPlant Physiology, 1973
- Isolation and characterization of a lysine-sensitive aspartokinase from a multicellular plantBiochemical and Biophysical Research Communications, 1970
- Regulation by methionine of the synthesis of third aspartokinase and of a second homoserine dehydrohenase in Escherichia coli K 12Biochimica et Biophysica Acta (BBA) - General Subjects, 1967
- Sequence of enzyme synthesis and gene replication during the cell cycle of Bacillus subtilis.Proceedings of the National Academy of Sciences, 1965