Intravenous Immunoglobulins Suppress Immunoglobulin Productions by Suppressing Ca2+ ‐Dependent Signal Transduction Through Fc γ Receptors in B Lymphocytes
- 1 July 1994
- journal article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 40 (1), 37-42
- https://doi.org/10.1111/j.1365-3083.1994.tb03430.x
Abstract
A high dose intravenous immunoglobulin (IVIG) therapy is used in the treatment of a wide range of autoimmune disorders. However, the mechanisms of the action of IVIGs remain poorly understood. To analyse the mechanisms of effects of IVIGs on immunoglobulin (Ig) production of B cells, the effects of IVIGs on B lymphoblastoid cell lines transformed by Epstein-Barr virus (LCLs) were investigated. The productions of IgG or IgM of LCLs were dose-dependently suppressed by polyethylene glycol (PEG)-treated IVIG or pH 4-treated IVIG though the productions were not or only slightly suppressed by pepsin-treated IVIG. The suppression by IVIGs was blocked by anti-human IgG Fc or anti-Fc gamma RII. C mu gene expression and mu s C terminal gene expression of LCLs were suppressed by PEG-treated IVIG, whereas neither C mu gene expression nor mu s C terminal gene expression of LCLs were suppressed by pepsin-treated IVIG. Although the increase in intracellular calcium concentration in LCLs was not suppressed by pepsin-treated IVIG, the increase was suppressed by PEG-treated IVIG. This suppressing effect of PEG-treated IVIG on intracellular calcium concentration of LCLs was blocked by anti-human IgG Fc or anti- Fc gamma RII. Our results suggest that IVIGs suppressed the Ca(2+)-dependent signal transduction through Fc gamma R on B-cell membrane, consequently, the transcription of C mu mRNA, especially secreted mu mRNA was suppressed in the B cells.Keywords
This publication has 25 references indexed in Scilit:
- The structure and function of Fc receptorsImmunology Today, 1988
- Structure and Function of Human and Murine Receptors for IgGAnnual Review of Immunology, 1988
- Calcium and inositolphosphates in the activation of T cell-mediated cytotoxicity.The Journal of Experimental Medicine, 1987
- Human leukocyte IgG Fc receptorsImmunology Today, 1986
- Crosslinking of surface immunoglobulin and Fc receptors on B lymphocytes inhibits stimulation of inositol phospholipid breakdown via the antigen receptors.The Journal of Experimental Medicine, 1985
- Competence of B cells for T-cell help in pokeweed mitogen-induced immunoglobulin productionClinical Immunology and Immunopathology, 1983
- Immunomodulating effect of human placenta‐eluted gamma globulins in rheumatoid arthritisArthritis & Rheumatism, 1982
- HIGH-DOSE INTRAVENOUS GAMMAGLOBULIN FOR IDIOPATHIC THROMBOCYTOPENIC PURPURA IN CHILDHOODThe Lancet, 1981
- Dysfunctions of Pokeweed Mitogen-stimulated T and B Lymphocyte Responses Induced by Gammaglobulin TherapyJCI Insight, 1981
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974