"Flavanone Synthase" in Oat Primary Leaves Time Course and Distribution at the Tissue and Subcellular Level

Abstract

“Flavanone Synthase” (FS) isolated from oat primary leaves shows maximal activity in the differentiating zone of five day old leaves. Activity of FS is distributed in both the upper and lower epidermis, but dominant in the mesophyll. After fractionation of mesophyll protoplasts of Avena by differential centrifugation, FS activity could only be detected in the “cytosol” and not in intact chloroplasts or other particulate fractions.