Mammalian U2 snRNP has a sequence-specific RNA-binding activity.
- 1 October 1989
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 3 (10), 1562-1571
- https://doi.org/10.1101/gad.3.10.1562
Abstract
The RNA branch formed during pre-mRNA splicing occurs at a wide variety of sequences (branch sites) in different mammalian pre-mRNAs. U2 small nuclear ribonucleoprotein (snRNP) binds to the pre-mRNA branch site following the interaction of a protein, U2AF, with the 3' splice site/polypyrimidine tract. Here we show that despite the variability of mammalian branch sites, U2 snRNP has a sequence-specific RNA-binding activity. Thus, RNA branch formation is regulated by two sequence-specific interactions: U2AF with the 3' splice site/polypyrimidine tract, and U2 snRNP with the branch site. The affinity of the branch site for U2 snRNP affects the efficiency of spliceosome assembly and splicing.This publication has 36 references indexed in Scilit:
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