Adaptation of Coenzyme Stimulation Assays for the Nutritional Assessment of Vitamins B1, B2 and B6 Using the Cobas Bio Centrifugal Analyser
- 1 January 1987
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 24 (1), 41-46
- https://doi.org/10.1177/000456328702400106
Abstract
Adaptation of coenzyme stimulation assays for the nutritional assessment of thiamine, riboflavin and pyridoxine on the Cobas Bio centrifugal analyser are described. Whole blood was collected into acid-citrate dextrose, which preserves the erythrocytes, prior to assay for several days. Washed erythrocytes stored at −70°C and subsequently thawed, showed altered enzyme activities. The methods offer improved precision over existing procedures and take advantage of the high throughput capabilities of the instrumentation.This publication has 9 references indexed in Scilit:
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