HISTOCHEMICAL DEMONSTRATION OF URIDINE DIPHOSPHATE GLUCOSE-GLYCOGEN TRANSFERASE IN ANIMAL TISSUES

Abstract

Polysaccharide was histochemically synthesized in muscle fibers and other tissue cells by using uridine diphosphate glucose as substrate. By revising the method previously described in light of new biochemical and histochemical findings more satisfactory optimal conditions were defined for the demonstration of the uridine diphosphate glucose-glycogen transferase enzyme. The histochemical properties of the reaction were very similar to those reported biochemically and this tended to verify the specificity of the reaction. Glucose-6-phosphate stimulated intensely the uridine diphosphate glucose-glycogen transferase reaction in an alkaline range.