Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope
- 22 May 1992
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5060), 1180-1184
- https://doi.org/10.1126/science.256.5060.1180
Abstract
Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.Keywords
This publication has 27 references indexed in Scilit:
- Large scale hexagonal domainlike structures superimposed on the atomic corrugation of a graphite surface observed by scanning tunneling microscopyLangmuir, 1991
- Graphite: A Mimic for DNA and Other Biomolecules in Scanning Tunneling Microscope StudiesScience, 1991
- Molecular-resolution images of Langmuir-Blodgett films and DNA by atomic force microscopyLangmuir, 1991
- Atomic-scale imaging of DNA using scanning tunnelling microscopyNature, 1990
- New scanning tunneling microscopy tip for measuring surface topographyJournal of Vacuum Science & Technology A, 1990
- Images of single-stranded nucleic acids by scanning tunnelling microscopyNature, 1989
- Scanning Tunneling Microscopy of Nucleic AcidsScience, 1989
- Tunneling microscopy study of the graphite surface in air and waterPhysical Review B, 1986
- Atomic Force MicroscopePhysical Review Letters, 1986
- An electron microscopic method for studying nucleic acid–protein complexes. Visualization of RNA polymerase bound to the DNA of bacteriophages T7 and T3Biopolymers, 1974