Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 283 (5750), 835-840
- https://doi.org/10.1038/283835a0
Abstract
A 5.1 kilobase-pair segment of rabbit chromosomal .beta.-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-.mu. yeast plasmid and the yeast leu-2 gene. S. cerevisiae transformed with the globin DNA-containing hybrid produced .beta.-globin-specific RNA. As compared to mature .beta.-globin mRNA, these transcripts lacked 20-40 nucleotides from the 5'' end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary .beta.-globin transcript could be detected in the yeast cells.Keywords
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