Charge transfer mediated by nigericin in black lipid membranes
- 1 February 1976
- journal article
- research article
- Published by Springer Nature in Journal of Bioenergetics and Biomembranes
- Vol. 8 (1), 19-26
- https://doi.org/10.1007/bf01559387
Abstract
Nigericin, in the concentration range (10−6M or higher) at which it uncouples intact mitochondria, was found to increase the conductance of black lipid membranes (BLM) by several orders of magnitude. The dependence of the membrane conductance on pH and K+ concentration suggests a mechanism for the transfer of charge mediated by this ionophore based on a mobile dimer with both nigericin molecules protonated and complexed with one K+. This charged complex accounts for the uncoupling effect observed in intact mitochondria.Keywords
This publication has 36 references indexed in Scilit:
- Model translocators for divalent and monovalent ion transport in phospholipid membranesThe Journal of Membrane Biology, 1974
- Complexes between uncouplers of oxidative phosphorylationThe Journal of Membrane Biology, 1974
- The mechanism of action of DNP on phospholipid bilayer membranesThe Journal of Membrane Biology, 1972
- Lipid-polypeptide interactions in bilayer lipid membranesThe Journal of Membrane Biology, 1972
- Ion Transport by Energy-Conserving Biological MembranesAnnual Review of Microbiology, 1971
- Thermodynamic consideration of the ion transporting antibioticsJournal of Molecular Biology, 1970
- Correlation between Anion Uptake and the Movement of K+ and H+ across the Mitochondrial MembraneEuropean Journal of Biochemistry, 1969
- Ion Transport Induced by Light and Antibiotics in Chromatophores from Rhodospirillum RubrumEuropean Journal of Biochemistry, 1968
- Effect of nigericin upon light-dependent monovalent cation transport in chloroplastsBiochemical and Biophysical Research Communications, 1967
- K+ — dependent uncoupling of photophosphorylation by nigericinBiochemical and Biophysical Research Communications, 1967