Activity of single ion channel proteins detected with a planar microstructure

Abstract

We present recordings of currents mediated by single ion channelproteins obtained using planar, microstructured glass chips. In these chips, pores with diameters of 1–2 μm are produced by ion track etching and are used for patch clamping instead of using the classical micropipette. Our results represent success in using such devices to record from single channels in cell membranes. The planar chip greatly enhances the accessibility of the ion channel containing membrane and can serve as a workbench for experiments on single ion channels using combinations of patch clamp current recording with other single molecule techniques.