Simple High Performance Liquid Chromatography Determination of Captopril in Human Plasma and Cerebrospinal Fluid
- 28 February 1989
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 12 (4), 629-643
- https://doi.org/10.1080/01483918908051763
Abstract
A simple HPLC assay has been developed for free captopril in plasma and CSF, and for its mixed disulphides in CSF. Captopril is derivatized with p-bromophenacylbromide (p-BPB), and the adduct formed is extraced and then assayed using a reversed-phase column and UV detection. Captopril mixed disulphides are reduced with tributylphosphine to the free form which in turn is trapped with p-BPB. No clean-up procedures are necessary for this method which shows satisfactory accuracy and precision. A sensitivity level of 10 ng/mL has been reached for free total captopril in CSF, and for free captopril in plasma.This publication has 18 references indexed in Scilit:
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