Clearing Specimens for the Demonstration of Bone
- 1 January 1939
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 14 (1), 7-11
- https://doi.org/10.3109/10520293909110285
Abstract
A brief review is given of some of the older technics of clearing and staining various types of animals, in such a way that the bones are clearly visible in the surrounding tissues. The authors have modified some of these earlier practices. Particularly good results were obtained by combining the Schultze technic with that of Lundvall. Embryos are fixed in 95% alcohol, treated with 1% KOH, stained in 110,000 alizarin Red S, cleared in glycerin, dehydrated with alcohol and further cleared in toluol, toluol saturated with naphthalcne, and anise oil saturated with naphthalcne. Details of the technic are presented in the text.This publication has 4 references indexed in Scilit:
- A Technic for Clearing Large InsectsStain Technology, 1936
- A Note on the Staining of the Skeleton of Cleared Specimens with Alizarin Red SStain Technology, 1926
- The differential staining of bone. I. The staining of preserved specimensThe Anatomical Record, 1921
- On ossification centers in human embryos less than one hundred days oldJournal of Anatomy, 1906