Radioimmunoassay of 18-hydroxy-ll-deoxycorticosterone in Plasma
- 1 October 1976
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 43 (4), 803-809
- https://doi.org/10.1210/jcem-43-4-803
Abstract
A method is described for the radioimmunoassay of 18-OH-DOC using antibodies generated in rabbits against the carboxymethoxime derivative coupled to bovine serum albumin. The procedure uses 4 ml of plasma with intra and interassay variations of 8 and 9% respectively. Standard 18-OH-DOC added to plasma from an adrenalectomized patient gave a regression equation, Y = 0.974 X ± 2.210 and a correlation coefficient of 0.999. The only cross reacting steroid, 18-OH-B, which may lead to falsely high levels is removed by a single thin layer chromatographic stepBlood levels in normal subjects agree closely with those calculated indirectly by metabolic clearance and secretion rate measurements. ACTH stimulation produced an 18-fold increase in plasma concentration while dexamethasone suppression decreased levels 3-fold. Four hours in the upright position resulted in a decreased plasma concentration while aldosterone increased. No significant response to dietary sodium restriction could be demonstrated.Keywords
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