Proximity of Reactive Lysyl Residue to the Antigenic Site in Rabbit Skeletal Myosin against the Monoclonal Antibody (MF-18) Generated to Chicken Skeletal Myosin1
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 95 (3), 721-727
- https://doi.org/10.1093/oxfordjournals.jbchem.a134662
Abstract
MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very close to the hapten site. This is consistent with the evidence that the hapten site resides in the 26, 000 dalton tryptic fragment of S1. Use of such antibodies as labels may open the way to determining the location of specific hapten sites in the three-dimensional image of actin-S1 complex reconstructed from the electron micrographs.Keywords
This publication has 2 references indexed in Scilit:
- Three-Dimensional Image Analysis of the Complex of Thin Filaments and Myosin Molecules from Skeletal Muscle. III. The Multi-Domain Structure of Actin-Heavy Meromyosin Complex1The Journal of Biochemistry, 1981
- Location of the Nonidentical Two Reactive Lysine Residues in the Myosin Molecule1The Journal of Biochemistry, 1981