The isolation and characterization of 60 nm vesicles (‘nanovesicles’) produced during ionophore A23187-induced budding of human erythrocytes

Abstract

In addition to the microvesicles released during the treatment of human erythrocytes with Ca2+ and ionophore A23187, a new subpopulation of still smaller dense vesicles (nanovesicles) has been identified. Nanovesicles are about 60 nm in diameter, have an acetylcholinesterase activity higher than that of microvesicles and appear to be relatively enriched in sphingomyelin and correspondingly depleted of phosphatidylethanolamine. They have a polypeptide composition different from those of erythrocyte membranes or microvesicles, consisting largely of components of MW 60,000 and 26,000 in addition to Hb. These 2 major polypeptides do not appear to represent contaminating cytoplasmic proteins or proteolytic subfragments of a larger protein.