Induction of tolerance to composite tissue allograft in a rat model
- 1 January 2006
- journal article
- Published by Wiley in Microsurgery
- Vol. 26 (8), 573-578
- https://doi.org/10.1002/micr.20297
Abstract
The goal of this study was to establish a rat model that can be used to determine the variables in influencing induction of tolerance to composite tissue allografts. An anti T‐cell depleting agent (R73) and 15‐deoxyspergualin were given in different doses and schedule to four groups of Lewis rats receiving a limb transplant from Brown‐Norway donors. Graft survival prolongation was maximal combining a single dose of R73 and a 20‐day administration of 15‐deoxyspergualin. Long‐term survivors accepted a skin graft from Brown‐Norway donors at 80 days, but rejected grafts from an unrelated donor. Skin grafting did not influence survival of the transplanted limb. Mixed allogeneic chimerism was not detectable in peripheral blood by flow cytometry, but immunohistochemistry identified donor‐derived cells in the thymus of tolerant recipients at 100 days. These results suggest a state of donor‐specific, dynamic tolerance, with potential for future application in human composite tissue allotransplantation. © 2006 Wiley‐Liss, Inc. Microsurgery, 2006Keywords
This publication has 27 references indexed in Scilit:
- Induction of donor-specific tolerance in rat hind-limb allografts under antilymphocyte serum and cyclosporine A protocolThe Journal of Hand Surgery, 2002
- What is happening with hand transplants?The Lancet, 2001
- Hand transplantation: Comparisons and observations of the first four clinical casesMicrosurgery, 2000
- Donor-specific tolerance induction in composite tissue allograftsThe American Journal of Surgery, 1998
- LONG-TERM COMPOSITE TISSUE ALLOGRAFT SURVIVAL IN A NOVEL PORCINE MODEL WITH CYCLOSPORINE (CSA)/MYCOPHENOLATE MOFETIL (MMF) THERAPYTransplantation, 1998
- The Effect of Combined Immunosuppression with Systemic Low-Dose Cyclosporin and Topical Fluocinolone Acetonide on the Survival of Rat Hind-Limb AllograftsAnnals of Plastic Surgery, 1994
- Tolerance, Danger, and the Extended FamilyAnnual Review of Immunology, 1994
- Relative Antigenicity of Components of a Vascularized Limb AllograftPlastic and Reconstructive Surgery, 1991
- Deoxyspergualin directly suppresses antibody formation in vivo and in vitro.The Journal of Antibiotics, 1990
- Suppression of tissue graft rejection by spergualin.The Journal of Antibiotics, 1985