Budvicia aquatica gen. nov., sp. nov.: a Hydrogen Sulfide-Producing Member of the Enterobacteriaceae

Abstract

Deoxyribonucleic acid relatedness studies (SI nuclease method) showed that 60 strains proposed as Budvicia aquatica form a homogeneous deoxyribonucleic acid hybridization group. Three strains labeled Budvicia-like were 9 to 22% related to B. aquatica. A total of 74 strains representing known species and genera in the Enterobacteriaceae were 0 to 8% related to B. aquatica. These findings support designation of Budvicia aquatica as a new genus and new species. This new species in the family Enterobacteriaceae is composed of strains which produce H2S; hydrolyze urea and o-nitrophenyl-β-D-galactopyranoside; do not produce acid from trehalose, D-mannose, glycerol, sucrose, maltose, and D-melibiose; do not decarboxylate lysine, ornithine, or arginine; do not produce phenylalanine deaminase; and have complex growth factor requirements. The guanine-plus-cytosine content of the deoxyribonucleic acid is 46 mol%. The type strain is strain 20186HG01 (= ATCC 25567).