p34cdc2 Kinase is localized to distinct domains within the mitotic apparatus

Abstract

Antibodies to both the C-terminal and the N-terminal regions of the 34 kd serinethreonine specific protein kinase, p34^cdc2, were used to study the distribution of this protein in dividing cells and isolated chromosomes of the Indian muntjac. p34^cdc2 was found to be present throughout the cytoplasm of dividing cells. In addition, a portion of cellular p34^cdc2 was localized to the centrosome, kinetochore, and intercellular bridge and along kinetochore-to-pole microtubules during cell division. Tubulin-denuded metaphase kinetochores retained their association with p34^cdc2. The detection of p34^cdc2 within a variety of domains of the mitotic apparatus, in addition to the previous reported association with the centrosome [Bailly et al., EMBO J. 8:3985–3995, 1989; Raibowol et al., Cell 57:393–401, 1989] suggests that p34^cdc2 may play a role in events associated with anaphases A and B as well as with the transition between interphase and mitosis.