Failure of defective interfering particles of Sindbis virus produced in BHK or chicken cells to affect viral replication in Aedes albopictus cells

Abstract

Whereas defective interfering particles of Sindbis virus are readily produced in BHK-21 [baby hamster kidney] cells or chicken embryo fibroblasts by the techniques of serial undiluted passage, similar methods failed to generate such particles in A. albopitus cell cultures. Sindbis virus stocks produced in BHK-21 cells or chicken embryo fibroblasts and which contained defective interfering particles, when tested in A. albopictus cells, failed to interfere with the replication of standard Sindbis virus and to change the pattern of intracellular viral RNA synthesis from that produced by infection with standard Sindbis virus alone. Defective interfering particles of Sindbis virus generated in chicken or hamster cells are apparently silent or inert in misquito cells.