Syntheses and applications of water-soluble reactive polymers for purification and immobilization of biomolecules
- 1 November 1989
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 34 (9), 1186-1190
- https://doi.org/10.1002/bit.260340909
Abstract
Reactive polymers have been prepared by copolymeriz-ing N-isopropyl acrylamide (NIPAM) with N-acryloxy-succinimide (NASI) or glycidyl methacrylate (GMA). The amino groups of ligands could react with the residues of NASI or GMA and the polymers could be precipitated by temperature and/or salinity variation, since they contained the NIPAM residues. As a model, p-aminobenza-midine, a trypsin inhibitor, was attached to the polymers to form water-soluble macroligands, capable of selectively binding trypsin from a trypsin–chymotrypsin solution. After precipitation of the macroligand–trypsin complex, followed by dissociation, approximately 82% trypsin was isolated. The NIPAM-GMA copolymer was also reacted with immunogammaglobulin (IgG) and alkaline phosphatase (AP). It was demonstrated that the IgG bearing polymer was able to bind protein A and the whole complex was precipitable. The reactive polymer was also used for direct immobilization of AP which was active in repeated reactions.This publication has 9 references indexed in Scilit:
- A continuous affinity ultrafiltration process for trypsin purificationBiotechnology & Bioengineering, 1988
- Studies on the application of a newly synthesized polymer for trypsin purificationEnzyme and Microbial Technology, 1987
- Preparation and characterization of urease immobilized on to collagen-poly (glycidyl methacrylate) graft copolymerBiotechnology & Bioengineering, 1984
- Ultrafiltration affinity purificationJournal of Chromatography A, 1984
- Protein A: nature's universal anti-antibodyTrends in Biochemical Sciences, 1982
- THE AFFINITY PRECIPITATION TECHNIQUEAnnals of the New York Academy of Sciences, 1981
- Biospecific ultrafiltration: A promising purification technique for proteins?Journal of Membrane Science, 1981
- The preparation and properties of two new chromogenic substrates of trypsinArchives of Biochemistry and Biophysics, 1961
- A spectrophotometric determination of trypsin and chymotrypsinBiochimica et Biophysica Acta, 1955