Inhibition of human cytomegalovirus replication by small interfering RNAs
Open Access
- 1 January 2004
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 85 (1), 179-184
- https://doi.org/10.1099/vir.0.19453-0
Abstract
Small interfering RNAs (siRNAs) are the mediators of a sequence-specific process of gene silencing called RNA interference (RNAi). Here, we show that synthetic siRNAs against essential gene products of human cytomegalovirus (HCMV) can trigger RNAi in serum-starved, infected primary fibroblasts, as well as in U373 cells, leading to effective inhibition of viral DNA replication. This opens new possibilities for antiviral strategies and for the analysis of viral and cellular genes important to HCMV physiology.Keywords
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