Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid
Open Access
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (9), 3627-3640
- https://doi.org/10.1093/nar/14.9.3627
Abstract
Self-cleavage of both plus and minus RNA transcripts of the 247-residue avocado sunblotch viroid(ASBV), prepared from tandem dimeric cONA clones, occurs specifically at two sites in each transcript to give monomeric plus and minus species.The cleavage reaction occurs both during transcription and on incubation of purified transcripts at pH 8 and 37°C in the presence of magnesium ions to give a 3'-terminal 2',3'-cyclic phosphate and a 5'-terminal hydroxyl group.Keywords
This publication has 29 references indexed in Scilit:
- VIROIDSAnnual Review of Biochemistry, 1985
- RNA splicing in neurospora mitochondria: Self-splicing of a mitochondrial intron in vitroCell, 1984
- A Replication Cycle for Viroids and Other Small Infectious RNA'sScience, 1984
- Self cleavage of a precursor RNA from bacteriophage T4Journal of Molecular Biology, 1984
- The RNA moiety of ribonuclease P is the catalytic subunit of the enzymeCell, 1983
- Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reactionNature, 1983
- Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymenaCell, 1982
- Cell-Free Circularization of Viroid Progeny RNA by an RNA Ligase from Wheat GermScience, 1982
- In vitro splicing of the ribosomal RNA precursor of tetrahymena: Involvement of a guanosine nucleotide in the excision of the intervening sequenceCell, 1981
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980