Requirement of Ginkgo Pollen-Derived Tissue Cultures for Boron and Effects of Boron Deficiency

Abstract
A new test system for studying the role of B in the metabolism of plants was employed, the use of sterile shake-cultures of Ginkgo pollen-derived tissue, which is made up of small masses of homogeneous parenchymatous cells. Two general effects of boron deficiency were found: reduced rate of cell division with no significant effect on cell size, and some alteration in composition of the cell walls. With the exception of a reduction in fructose, there was no effect of boron deficiency on the concentration of soluble and of readily hydrolyzable carbohydrates, nor on the concentration of protein in the tissue.