EVIDENCE THAT BINDING OF 7,12-DIMETHYLBENZ(A)ANTHRACENE TO DNA IN MOUSE EMBRYO CELL-CULTURES RESULTS IN EXTENSIVE SUBSTITUTION OF BOTH ADENINE AND GUANINE RESIDUES
- 1 January 1983
- journal article
- research article
- Vol. 43 (9), 4132-4135
Abstract
Primary mouse embryo cell cultures were grown in the presence of [14C]guanine, labeling primarily deoxyguanosine residues in the cellular DNA, or in the presence of [14C]adenine, labeling both deoxyguanosine and deoxyadenosine residues in the cellular DNA. These culltures were subsequently exposed to 7,12-[3H]dimethylbenz(a)anthracene for 24 h. The DNA was isolated and hydrolyzed to deoxyribonucleosides, and the 7,12-dimethylbenz(a)anthracene:deoxyribonucleoside adducts were separated chromatographically allowing the 3 major adducts to be identified as bay-region anti-dihydrodiol-epoxide:deoxyguanosine and :deoxyadenosine adducts, and a bay-region syn-dihydrodiol-epoxide:deoxyadenosine adduct. In contrast to what is known for benzo(a)pyrene, substantial amounts of deoxyadenosine adducts are formed with the more potent carcinogen, 7,12-dimethylbenz(a)anthracene.This publication has 11 references indexed in Scilit:
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