First‐trimester prenatal diagnosis of cystic fibrosis using the polymerase chain reaction: Report of eight cases
- 1 May 1989
- journal article
- research article
- Published by Wiley in Prenatal Diagnosis
- Vol. 9 (5), 349-355
- https://doi.org/10.1002/pd.1970090508
Abstract
Eight pregnancies at risk for cystic fibrosis have been monitored by first‐trimester prenatal diagnosis with DNA amplification analysis. The polymerase chain reaction (PCR) was used in all cases to amplify the region detected by KM 19. In two cases, the region detected by CS·7, another DNA probe tightly linked to the CF locus, was also examined. The results of the PCR determinations were confirmed using the Southern blotting procedure, by segregation analysis of restriction fragment length polymorphisms (RFLPs) relative to XV‐2c, J3·11, metH, metD, and KM19 probes.Keywords
This publication has 18 references indexed in Scilit:
- SAME-DAY, FIRST-TRIMESTER ANTENATAL DIAGNOSIS FOR CYSTIC FIBROSIS BY GENE AMPLIFICATIONThe Lancet, 1988
- PRENATAL DIAGNOSIS OF CYSTIC FIBROSIS BY DNA AMPLIFICATION FOR DETECTION OF KM-19 POLYMORPHISMThe Lancet, 1988
- Prenatal diagnosis of cystic fibrosis.Archives of Disease in Childhood, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- Patterns of polymorphism and linkage disequilibrium for cystic fibrosisGenomics, 1987
- An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA SequencesNew England Journal of Medicine, 1987
- A candidate for the cystic fibrosis locus isolated by selection for methylation-free islandsNature, 1987
- FIRST-TRIMESTER PRENATAL DIAGNOSIS OF CYSTIC FIBROSIS WITH LINKED DNA PROBESThe Lancet, 1986
- A polymorphic DNA marker linked to cystic fibrosis is located on chromosome 7Nature, 1985
- PROSPECTIVE PRENATAL DIAGNOSIS OF CYSTIC FIBROSISThe Lancet, 1985