Cu(I) recognition via cation-π and methionine interactions in CusF
- 23 December 2007
- journal article
- research article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 4 (2), 107-109
- https://doi.org/10.1038/nchembio.2007.57
Abstract
Methionine-rich motifs have an important role in copper trafficking factors, including the CusF protein. Here we show that CusF uses a new metal recognition site wherein Cu(I) is tetragonally displaced from a Met2His ligand plane toward a conserved tryptophan. Spectroscopic studies demonstrate that both thioether ligation and strong cation-π interactions with tryptophan stabilize metal binding. This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry.Keywords
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