Sensitivity of the T. Pallidum Immobilization (TPI) TestA Function of the Number of Spirochaetes in the Antigen
Open Access
- 1 December 1958
- journal article
- research article
- Published by BMJ in Sexually Transmitted Infections
- Vol. 34 (4), 246-249
- https://doi.org/10.1136/sti.34.4.246
Abstract
The TPI test is most sensitive when there is only a single treponeme per high dry darkfield, and sensitivity decreases when 5 or 15 treponemes are present per high dry darkfield. This is evidenced both by a decrease in titer, and by rendering doubtful or negative results in tests which are positive when there is only a single treponeme per darkfield. Since the routine use of an antigen containing only one treponeme per darkfield is impractical, dilution of the antigen to 5 treponemes per darkfield is advised. If a doubtful reaction is obtained with this test, repeat the test with only a single treponeme per darkfield.Keywords
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