Incompatibility repressor in a RepA-like replicon of the IncFI plasmid ColV2-K94

Abstract

The replication region Rep1 of the IncFI plasmid ColV2-K94 was cloned on self-replicating restriction fragments. Rep1 was structurally and functionally homologous to the RepA replicon of IncFII R plasmids. Despite this close relationship, these two replication systems were compatible with each other. The nucleotide sequence of the copA incompatibility-replication control gene of Rep1 was determined and compared with the copA sequence of RepA. Six base changes were found in a 24-base-pair span of the copA gene; these may result in the formation of a new, more stable, 49-base stem-loop structure in the potential CopA RNA repressor molecule. We postulate that these alterations weaken the interaction between RNA transcripts of the Rep1 and RepA replicons.