INHIBITION BY 6-MERCAPTOPURINE OF PURINE PHOSPHORIBOSYLTRANSFERASES FROM EHRLICH ASCITES-TUMOUR CELLS THAT ARE RESISTANT TO THIS DRUG

Abstract

A strain of Ehrlich ascites-tumor cells that showed little inhibition of growth in the presence of 6-mercaptopurine accumulated less than 5% as much 6-thioinosine 5[image]-phosphate in vivo, in the presence of 6-mercaptopurine, as did the sensitive strain from which it was derived. Specific activities of the phosphoribosyltransferases that convert adenine, guanine, hypoxanthine and 6-mercaptopurine into AMP (adenosine monophosphate), GMP (guanosine monophosphate), IMP (inosine mono-phosphate) and 6-thioinosine 5[image]-phosphate were similar in extracts of the resistant and the sensitive cells. As found previously with sensitive cells, 6-mercaptopurine is a competitive inhibitor of guanine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase from the resistant cells and does not inhibit the adenine phosphoribosyltransferase from these cells. Michaelis constants and inhibitor constants of the purine phosphoribosyltransferases from resistant cells did not differ significantly from those measured with the corresponding enzymes from sensitive cells. Resistance to 6-mercaptopurine in this case is probably not due to qualitative or quantitative changes in these trans-ferases.