Immunochemical Detection of Tumor-Specific and Embryonic Antigens of Diethylnitrosamine-Induced Guinea Pig Tumors2

Abstract

Xenogeneic antibodies against chemically induced transplantable guinea pig hepatoma cells (line-1 and line-10) were obtained by immunization of rabbits with live cells. By appropriate absorption of antisera we obtained antibody against tumor-specific antigen. The specificity of the absorbed antisera was shown in 3 different tests: C1 fixation and transfer, fluorescent antibody, and complement- dependent cytotoxicity. Analysis of absorption results with normal adult guinea pig tissues and guinea pig embryo showed that in addition to line-specific tumor antigens, line-1 and line-10 tumor cells have an embryonic antigen in common. Using specific antibody, we found that the minimum number of line-10-specific tumor antigen sites/cell was about 100,000. The xenogeneic antisera were also used in an antibody absorption test for measurement of soluble tumor-specific antigen.