PCR‐based identification of wheat genomes
- 1 August 1995
- journal article
- Published by Wiley in Molecular Ecology
- Vol. 4 (4), 509-514
- https://doi.org/10.1111/j.1365-294x.1995.tb00246.x
Abstract
We describe a PCR system that distinguishes the A, B and D genomes in wheat DNA extracts. PCRs were directed at the 'non-transcribed spacer' regions of the rDNA loci. The spacers within the D genome locus have a 71-bp insertion that is absent from the corresponding A and B loci. PCR product sizes therefore enable D- and D+ genomes to be distinguished. The A and B genomes can be differentiated by PCR with an internal primer which does not anneal to A genome sequences. This work is relevant to the ancient ecology of wheat, as it is often difficult to determine ploidy level from morphological examination of archaeobotanical remains.Keywords
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