Histobacteriology of the Genus Pasteurella II. Specificity and Stability Of the Various Species of Pasteurella

Abstract

Summary Antisera prepared against Pasteurella anatipestijer, P. gallinarum, P. haemolytica, P. multocida (polyvalent) were specific for the species against which they were produced. A cross reaction was noted between P. tularensis and P. novicida which could be eliminated by diluting either antiserum 1:5. Polyvalent antiserum produced against P. pseudotuberculosis stained strains of P. pestis deficient in Fraction 1 and species of Salmonella containing either somatic factors 4 and 9. The antigens of various species of the genus Pasteurella differ in their reactions with their antibodies after exposure to chemicals used routinely in histological processing. P. anatipestifer, P. gallinarum, P. haemolytica, P. multocida, P. novicida, and P. tularensis retain their ability to react with species specific antisera after treatment, while P. pestis and P. pseudotuberculosis are rendered non-reactive by most chemicals used in tissue embedding techniques.