PRELIMINARY INVESTIGATIONS INTO THE NATURE OF NEUTRAL 17-KETOSTEROIDS IN HUMAN PLASMA*
- 1 June 1955
- journal article
- research article
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 15 (6), 693-701
- https://doi.org/10.1210/jcem-15-6-693
Abstract
A pool of 950 ml. of plasma from 4 healthy adult males was examined for neutral 17-ketosteroid content. The procedure involved extraction of the steroid with ether-ethanol, hydrolysis of the 17-ketosteroid conjugates, and purification by solvent partitions and Girard separation. The crude neutral fraction was chromato-graphed on alumina. Dehydroepiandrosterone and androsterone were identified, the former constituting 65% and the latter, 11% of the total neutral ketonic fraction. Trace amounts of unidentified 17-ketosteroids were found.Keywords
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