Refinement of Fluorescent Antibody by Gel Filtration.

Abstract

Gel filtration through a Sephadex (Pharmacia, Uppsala, Sweden) column permits a significantly simpler and more rapid purification of fluorescent conjugates than either anion-exchange or electrophoresis. Dialysis was eliminated and unconjugated dye as well as other low molecular weight fractions were separated with minimum loss of protein or titer. Detailed data on purification of some anti-fungal conjugates are presented. Ethodin extraction of serum antibody preparatory to conjugation and gel filtration further expedites the process.