A METHOD FOR THE ESTIMATION OF OESTRIOL, OESTRONE AND OESTRADIOL-17β IN THE BLOOD OF THE PREGNANT WOMAN AND OF THE FOETUS
Open Access
- 1 September 1960
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 21 (1), 9-23
- https://doi.org/10.1677/joe.0.0210009
Abstract
SUMMARY: A chemical method for the estimation of oestriol, oestrone and oestradiol-17β in the blood of the pregnant woman and of the foetus is described. It is based on a method previously developed for urine and involves acid hydrolysis of whole blood, extraction of the phenolic fraction, methylation, chromatography on alumina columns and colorimetric measurement using a micro-modification of the Kober reaction. Kober-chromogenic impurities in the final extracts are derived from the procedure, not from the blood, and are eliminated by the spectrophotometric correction of Allen. The accuracy, precision, sensitivity and specificity of the method have been determined.Keywords
This publication has 13 references indexed in Scilit:
- A Comparison of Methods for the Analysis of Estrone, Estradiol, and Estriol in Extracts of Human UrineJournal of Biological Chemistry, 1958
- AN EVALUATION OF A CHEMICAL METHOD FOR THE ESTIMATION OF OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINEJournal of Endocrinology, 1957
- AN ADDITIONAL PURIFICATION STEP FOR A METHOD FOR ESTIMATING OESTRIOL, OESTRONE AND OESTRADIOL-17β IN HUMAN URINEJournal of Endocrinology, 1957
- Studies on Phenolic Steroids in Human Subjects. II. The Metabolic Fate and Hepato-Biliary-Enteric Circulation of C14-Estrone and C14-Estradiol in Women1Journal of Clinical Investigation, 1957
- A method for the determination of oestriol, oestrone and oestradiol-17β in human urine by partition chromatography and colorimetric estimationBiochemical Journal, 1956
- ISOLATION AND ESTIMATION OF »FREE« OESTROGENS IN HUMAN PLACENTAEActa Endocrinologica, 1956
- A chemical method for the determination of oestriol, oestrone and oestradiol in human urineBiochemical Journal, 1955
- A CHEMICAL METHOD FOR THE DETERMINATION OF ESTROGENS IN PLASMAJournal of Biological Chemistry, 1953
- CONVERSION OF ESTRONE ON INCUBATION IN BLOODJournal of Biological Chemistry, 1950
- A SIMPLE METHOD FOR ANALYZING COMPLICATED ABSORPTION CURVES, OF USE IN THE COLORIMETRIC DETERMINATION OF URINARY STEROIDSJournal of Clinical Endocrinology & Metabolism, 1950