Collaborative Study of a Method for Chemical Confirmation of the Identity of Aflatoxin

Abstract

The chemical method for confirmation of the identity of aflatoxin by derivative formation directly on the TLC plate was studied collaboratively by 8 participants. The results show that aflatoxin B₁ was confirmed in 17 of 17 sample extracts representing 15 μg aflatoxin B₁/kg peanut butter, in 13 of 16 extracts representing 5 μg/kg, and in none of the 7 aflatoxin-free extracts. Collaborators commented that the method was easily performed and gave good results. The method has been adopted as official first action.