DNA sequence and characterization of human class II major histocompatibility complex beta chains from the DR1 haplotype.
- 1 May 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (10), 3405-3409
- https://doi.org/10.1073/pnas.82.10.3405
Abstract
Two HLA class II .beta.-chain clones from a cell line homozygous for the DR1 haplotype have been characterized and sequenced. They represent a DR.beta. chain (2918.4) and a DQ.beta. chain (2918.8). Clone 2918.4 has been used to select mRNA from a lymphoblastoid cell line, and this was injected into Xenopus oocytes with mRNA selected with a DR.alpha. chain. The translation products were immunoprecipitated with a .beta.-chain-specific monoclonal antibody and electrophoresed on 2-dimensional gels. This revealed positive signals in the positions predicted for .beta. and .alpha. chains. Sequence comparisons of 2918.4 with previously published DR.beta.-chain sequences confirm the presence of 2 regions of variability in the membrane distal domain. Analysis of the sequence of 2918.8 identified it as a DQ.beta. chain identical to one previously published from a DR3,w6 cell line. The DQ.beta. sequence apparently represents the DQ1 specificity shared by the DR1 and DRw6 haplotypes.This publication has 18 references indexed in Scilit:
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