Interphase nuclear matrix and metaphase scaffolding structures
- 1 January 1984
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 1984 (Supplement), 103-122
- https://doi.org/10.1242/jcs.1984.supplement_1.8
Abstract
The protein compositions of purified metaphase chromosomes, nuclei and their residual scaffold and matrix structures, are reported. The protein pattern of nuclei on sodium dodecyl sulphate/polyacrylamide gels is considerably more complex and rich in non-histone proteins than that of chromosomes. Nuclei contain about three to four times more non-histone proteins relative to their histones than chromosomes. Besides the protein components of the peripheral lamina, several protein bands are specific or at least highly enriched in nuclei. Conversely, two proteins X0 (33×103 Mr) and X1 (37×103 Mr) are highly enriched in the pattern of metaphase chromosomes.Keywords
This publication has 54 references indexed in Scilit:
- Immunological localization of the major architectural protein associated with the nuclear envelope of the Xenopus laevis oocyteExperimental Cell Research, 1982
- Non-histone proteins and long-range organization of HeLa interphase DNAJournal of Molecular Biology, 1982
- RNA is synthesized at the nuclear cageNature, 1981
- Considerations in the isolation of rat liver nuclear matrix, nuclear envelope, and pore complex laminaExperimental Cell Research, 1981
- Enzyme-bound early product of purified poly(ADP-ribose) polymeraseBiochemical and Biophysical Research Communications, 1977
- Identification of a nuclear protein matrixBiochemical and Biophysical Research Communications, 1974
- Synthesis of 5 S and 4 S RNA in Metaphase-Arrested HeLa CellsScience, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- RIBONUCLEIC ACID AND PROTEIN SYNTHESIS IN MITOTIC HELA CELLSThe Journal of cell biology, 1965
- Nicotinamide mononucleotide activation of a new DNA-dependent polyadenylic acid synthesizing nuclear enzymeBiochemical and Biophysical Research Communications, 1963