Influence of sample quantification and taxonomic resolution on the ordination of macroinvertebrate communities from running waters in Victoria, Australia

Abstract

The influence of sample quantification and taxonomic resolution on the ordination of macroinvertebrate communities from nine Victorian rivers was examined by progressively reducing the degree of detail in the original data (species level, quantitative). Five additional data sets were created that consisted of binary (presence or absence) data on species, quantitative or binary data on families, and quantitative data on PET (plecopteran, ephemeropteran and trichopteran) species or families. Ordinations were performed with detrended correspondence analysis (DCA) and semi-strong hybrid multi-dimensional scaling (SSH). With both ordination techniques, the ordinations of each data set (including the original) revealed the same three underlying gradients. An altitudinal gradient consistently achieved the highest correlations with the ordinations (r = 0.71-0.93), followed by a substratum gradient (r = 0.50-0.88) and a combined pH and conductivity gradient (r = 0.47-0.76). Each of the five less-complete data sets thus provides an adequate degree of detail for ordination analysis and subsequent interpretation of environmental gradients.