ATP stimulates the uptake of S‐dinitrophenylglutathione by rat liver plasma membrane vesicles
- 21 November 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 240 (1-2), 55-58
- https://doi.org/10.1016/0014-5793(88)80339-9
Abstract
Incubation of inverted plasma membrane vesicles from rat liver with micromolar concentrations of S-dinitrophenylglutathione (DNP-SG) in the presence of ATP resulted in the uptake of DNP-SG into the vesicles. ATP-dependent DNP-SG accumulation was half-maximal with 9 μM DNP-SG, while the K m for ATP was 320 μM. Glutathione disulfide (GSSG), but not reduced glutathione, inhibited the ATP-dependent accumulation of DNP-SG by the vesicles, suggesting that the same, ATP-dependent transport system is responsible for the extrusion of glutathione conjugates and GSSG from liver cells.Keywords
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