Studies on lipogenesis in vivo: Comparison of cholesterol and fatty acid synthesis in rats and mice
- 1 March 1967
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 102 (3), 864-869
- https://doi.org/10.1042/bj1020864
Abstract
1. The importance of fatty acid synthesis as a pathway for the disposal of ingested glucose has been evaluated in rats and mice given a purified diet high in glucose and low in fat. [U-(14)C]Glucose was either added to the diet and fed for 24hr. or given by stomach tube as a 250mg. (mice) or 1000mg. (rats) meal. The two methods of isotope administration gave similar results. 2. Under the conditions employed fatty acid synthesis appeared to be a more important pathway for glucose disposal in mice than in rats. In mice 15.3% of ingested [U-(14)C]glucose was converted into fatty acid and in rats the corresponding value was 8.6%. In contrast, the conversion of [U-(14)C]glucose into cholesterol, as a percentage of dose, was twice as high in rats as in mice. 3. The effect of 20% of corn oil in the diet on the conversion of dietary [U-(14)C]glucose into fat was also investigated. Mice given diets containing 1% or 20% of corn oil converted 14.6% or 7.0% respectively of dietary [U-(14)C]glucose into fatty acid over a 24hr. period. There was no effect of fat on the incorporation of the isotope into cholesterol. 4. In mice given diets containing 1% or 20% of corn oil approx. 10% and 2% respectively of newly synthesized fatty acids were found in the liver. Hepatic fatty acid synthesis appears to be more sensitive to dietary fat than is extrahepatic synthesis.This publication has 10 references indexed in Scilit:
- Studies in lipogenesis in vivo. Fatty acid and cholesterol synthesis during starvation and re-feedingBiochemical Journal, 1966
- Studies on lipogenesis in vivo. Effects of starvation and re-feeding, and studies on cholesterol synthesisBiochemical Journal, 1966
- Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesized fatBiochemical Journal, 1966
- Lipogenesis and Enzyme Activity in Rat and Mouse Epididymal Adipose TissueExperimental Biology and Medicine, 1966
- FATTY ACID AND GLYCERIDE GLYCEROL SYNTHESIS FROM GLUCOSE DURING HIGH RATES OF GLUCOSE UPTAKE IN THE INTACT RATCanadian Journal of Biochemistry, 1965
- FATTY ACID SYNTHESIS IN NORMAL AND COLD-ACCLIMATED RATSCanadian Journal of Physiology and Pharmacology, 1964
- Biosynthesis of fatty acids and cholesterol as related to diet fatArchives of Biochemistry and Biophysics, 1963
- Some aspects of glucose metabolism in normal and alloxan-diabetic ratsBiochimica et Biophysica Acta, 1962
- [Research on the synthesis of fats from acetate or glucose. IV. Importance of the liver in lipogenesis; experimental analysis].1958
- Lipogenesis From Carbohydrate in the Negative Caloric Balance State Induced by Exposure to ColdAmerican Journal of Physiology-Legacy Content, 1957