Catalytic activity of α-chymotrypsin in which histidine-57 has been methylated
- 1 August 1971
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 124 (1), 13-18
- https://doi.org/10.1042/bj1240013
Abstract
The properties of a derivative of α-chymotrypsin in which histidine-57 has been methylated have been examined. Although the modified enzyme binds substrate with the same affinity as does native α-chymotrypsin, acylation and deacylation occur at much decreased rates. As for native α-chymotrypsin, a basic group of pKa approx. 7 is involved in both acylation and deacylation. The significance of these results is considered in relation to the normal function of histidine-57.Keywords
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