Utility and sensitivity of anti BrdU antibodies in assessing S‐phase cells compared to autoradiography

Abstract

A rapid and convenient method for estimating S‐phase cells in a population was developed which detects bromodeoxyuridine (BrdU) incorporation into DNA by means of monoclonal anti‐BrdU antibodies. This immunofluorescence technique (RPMB technique) was compared to autoradiographic (ARG) detection of tritiated thymidine (³HTdr) grains incorporated into the DNA. Using incubation periods for BrdU and ³HTdr ranging from one minute to one hour and detecting their incorporation by ARG and RPMB techniques, it became apparent that the RPMB technique was far more sensitive than ARG in addition to being extremely easy to perform. Some possible utilities of the RPMB technique are discussed.