COLLABORATIVE STUDY ON ANTIGENS FOR IMMUNODIAGNOSIS OF SCHISTOSOMIASIS
- 1 January 1982
- journal article
- research article
- Vol. 60 (5), 729-753
Abstract
Eight research laboratories in Europe and the USA were selected, on the basis of having published data on Schistosoma mansoni and S. japonicum antigens, to participate in a study of various antigen/test combinations for immunodiagnosis of schistosomiasis. The serum bank consisted of 395 well documented sera from 4 endemic areas in Brazil (2 areas), Kenya and the Philippines. Altogether, 21 S. mansoni and 4 S. japonicum antigen and immunoassay combinations were evaluated. S. mansoni egg antigens yielded a higher combined sensitivity than adult worm antigens, irrespective of their purity, in active S. mansoni infections before and after specific treatment. Quantitative seroreactivity of characterized S. mansoni egg antigens showed good correlation with fecal egg counts in the 5-14 yr age group. No correlation between morbidity related to S. mansoni and seroreactivity was observed in any test system. Three S. japonicum egg antigens showed high sensitivity and specificity in relation to the presence or absence of eggs in the stool. The quantitatives seroreactivity of the characterized S. japonicum egg antigens correlated directly with the intensity of S. japonicum infection in all age groups. The enzyme-linked immunosorbent assay (ELISA), using several different procedures, performed well with the antigens used in the study. The indium slide immunoassay (ISI), a simple qualitative visual test system using an S. mansoni egg antigen, demonstrated a high degree of sensitivity and specificity. The results did not indicate the superiority of any particular immunodiagnostic method for detecting antischistosome antibodies. This may be a 1st step towards developing and standardizing antigens for immunodiagnosis of schistosomiasis.This publication has 39 references indexed in Scilit:
- DETECTION BY IMMUNOFLUORESCENCE OF ANTIBODIES TO PARASITIC AGENTS. USE OF CLASS-SPECIFIC CONJUGATESAnnals of the New York Academy of Sciences, 1975
- THE RAISON D'ETRE OF STANDARDS IN INDIRECT IMMUNOFLUORESCENCEAnnals of the New York Academy of Sciences, 1975
- Are there stage-characteristic immunofluorescence patterns in schistosomiasis?Transactions of the Royal Society of Tropical Medicine and Hygiene, 1975
- A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni.1972
- Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GImmunochemistry, 1971
- DELAYED HYPERSENSITIVITY-TYPE GRANULOMA FORMATION AND DERMAL REACTION INDUCED AND ELICITED BY A SOLUBLE FACTOR ISOLATED FROM SCHISTOSOMA MANSONI EGGSThe Journal of Experimental Medicine, 1970
- IMMUNOLOGICAL STUDIES IN VARIOUS TYPES OF SCHISTOSOMIASISAnnals of the New York Academy of Sciences, 1969
- [Antigenic structure of helminthes. Immunological aspects of the host-parasite relationship].1968
- The Circumoval Precipitin (COP) Test in Schistosomiasis Japonica *The American Journal of Tropical Medicine and Hygiene, 1968
- DIAGNOSIS OF ALLERGY BY AN IN-VITRO TEST FOR ALLERGEN ANTIBODIESThe Lancet, 1967