Isolation of Intact Chloroplasts from Spinach Leaf by Centrifugation in Gradients of the Modified Silica “Percoll”
- 1 October 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 43 (10), 2137-2142
- https://doi.org/10.1080/00021369.1979.10863766
Abstract
The isolation of the photosynthetically competent chloroplast preparations was undertaken by means of the density gradient centrifugation on the modified silica sol “Percoll.” A clear separation of the intact chloroplast sustaining the high photosynthetic activities (light dependent CO2 fixation ca. 130μmol/mg Chl·hr) was established. The contamination of mitochondria and peroxisomes was estimated to be less than 3% by measuring the activities of their marker enzymes. The chloroplasts were proved to be free from endoplasmic reticulum and cytosol. The photosynthetic CO2 fixation of the isolated chloroplast preparations was saturated by illumination of the light intensity of 20,000 Lux (12 mW/cm2, 400~750 nm).This publication has 8 references indexed in Scilit:
- Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylaseProceedings of the National Academy of Sciences, 1978
- Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloidal Silica GradientsPublished by Springer Nature ,1977
- Synthesis of Proteins by Isolated Euglena gracilis ChloroplastsPlant Physiology, 1976
- Isolation of Intact Chloroplasts and Other Cell Organelles from Spinach Leaf ProtoplastsPlant Physiology, 1976
- Compartmentation and Transport in C4 PhotosynthesisPublished by Springer Nature ,1976
- ENZYMATIC CARBOXYLATION OF PHOSPHOENOLPYRUVATE .I. PURIFICATION AND PROPERTIES OF PHOSPHOENOLPYRUVATE CARBOXYLASE1966
- Correlation between Photosynthetic Activity and Membrane Integrity in Isolated Pea ChloroplastsPlant Physiology, 1965
- CatalasePublished by Elsevier ,1965