Mechanism of Inactivation of Enzyme Proteins by Ultraviolet Light
- 22 September 1961
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 134 (3482), 836-837
- https://doi.org/10.1126/science.134.3482.836
Abstract
Some quantum yields for the destruction of amino acids have been determined. The inactivation of the enzymes hymotrypsin, lysozyme, ribonuclease, and trypsin by ultraviolet light can be accounted for quantitatively by summing the products of (i) the probability that light is absorbed by a given amino acid residue, ε4, and (ii) the probability that absorbed light induces a chemical change, with a quantum efficiency φ4, in the residue. The principal residues involved are cystyl and tryptophanyl. Peptide bond rupture is not important. Analysis of inactivated enzymes verifies the assumption of the existence of several inactivation mechanisms.Keywords
This publication has 4 references indexed in Scilit:
- Effect of Ultraviolet Light Irradiation on the Peptide Bonds of ProteinBulletin of the Agricultural Chemical Society of Japan, 1959
- The action of monochromatic ultaviolet light on proteinsBiochimica et Biophysica Acta, 1957
- Photooxidation of crystalline ribonuclease in the presence of methylene blueArchives of Biochemistry and Biophysics, 1955
- The kinetics of the photochemical inactivation-denaturation of enzymes, viruses and related substancesArchives of Biochemistry and Biophysics, 1951