Connections of the corpus cerebelli in the thornback guitarfish,Platyrhinoidis triseriata (Elasmobranchii): A study with WGA-HRP and extracellular granule cell recording

Abstract

The neuronal connections of the cerebellar corpus in the guitarfish Platyrhinoidis triseriata were investigated by WGA‐HRP injections and extracellular recording of sensory evoked electrical activity. Injections of WGA‐HRP into the corpus resulted in retrograde labeling of the following cell groups bilaterally: pretectal and accessory optic nuclei, interstitial nucleus of Cajal, nucleus ruber, oculomotor and possibly trochlear nucleus, central (periaqueductal) gray, nucleus H, reticular formation of the midbrain, cerebellar nucleus, caudal part of nucleus R tentatively locus coeruleus and subcoeruleus field, octaval and trigeminal nuclei, intermediate octavolateralis nucleus, medial inferior reticular formation, lateral reticular nucleus, and spinal cord. Unilaterally labeled cells were seen in the contralateral inferior olive, which was found to project in. sagittal zones onto the molecular layer of the corpus. Terminal fields of efferent Purkinje cell axons were labeled over the ipsilateral cerebellar nucleus exclusively. Purkinje cells in different parts of the corpus project topographically onto subdivisions of the nucleus. Mapping of evoked electrical multiple unit activity recorded from the granule cell layer of the corpus shows separate visual and tactile areas, mostly confined to the anterior and posterior lobes, respectively. Granule cells within the tactile area also responded to lateral line stimuli and, at two distinct medial locations in the caudal and rostral parts of the posterior lobe, to weak electric field stimulation in the bath. The body surface is somatotopically represented in the tactile area, but discontinuities in the map might indicate that the somatotopy is “fractured.”